Cloning gene of interest in ecoli
Posted: Tue May 29, 2018 9:42 amHi
This is a quick question. I am new to the field of molecular Biology. Recently I inoculated a colony of ecoli that I obtained after transformation in 4ml of Luria broth media for 16 hours, did a miniprep using 3ml of the media and sequenced the DNA. The sequencing result is positive and it is confirmed that my gene of interest is present in the vector. My question is, how can I sure that every ecoli cell in the remaining 1ml of the media has the same positive sequence. Do mutations happen when ecoli replicate?
Please help.
I didn't find the right solution from the Internet.
References:-
http://forums.biotechniques.com/viewtop ... =5&t=44191
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This is a quick question. I am new to the field of molecular Biology. Recently I inoculated a colony of ecoli that I obtained after transformation in 4ml of Luria broth media for 16 hours, did a miniprep using 3ml of the media and sequenced the DNA. The sequencing result is positive and it is confirmed that my gene of interest is present in the vector. My question is, how can I sure that every ecoli cell in the remaining 1ml of the media has the same positive sequence. Do mutations happen when ecoli replicate?
Please help.
I didn't find the right solution from the Internet.
References:-
http://forums.biotechniques.com/viewtop ... =5&t=44191
Creative video advertising quote
Thanks