Crystallisation of protein with 2-propanol
Posted: Tue May 29, 2018 9:41 amHi everyone,
I am wondering if anyone could give me some advice or insight. Last year I began crystallising my protein of interest and luckily I was successful in obtaining crystals in a HEPES, 3-Na-citrate, 2-propanol condition although unfortunately the diffraction quality was poor. The crystals are quite small and very tedious to fish. I have tried streak seeding and the crystals appeared similar. I am not a crystallographer in my research and would appreciate any insight as I would like this to use it to compliment a chapter in PhD Thesis.
Please help.
I didn't find the right solution from the Internet.
References:-
http://forums.biotechniques.com/viewtop ... =5&t=44371
motion graphics video quote
Thanks
I am wondering if anyone could give me some advice or insight. Last year I began crystallising my protein of interest and luckily I was successful in obtaining crystals in a HEPES, 3-Na-citrate, 2-propanol condition although unfortunately the diffraction quality was poor. The crystals are quite small and very tedious to fish. I have tried streak seeding and the crystals appeared similar. I am not a crystallographer in my research and would appreciate any insight as I would like this to use it to compliment a chapter in PhD Thesis.
Please help.
I didn't find the right solution from the Internet.
References:-
http://forums.biotechniques.com/viewtop ... =5&t=44371
motion graphics video quote
Thanks