p38 difficult to express??
Posted:
Thu Jan 05, 2012 7:36 pmby meltoumi
Anyone else find MAPK14 or p38 alpha difficult to recombinantly express at high titer levels? When p38 is folded and active, it seems to slow cell life cycle and decrease expression. Any thoughts?
Re: p38 difficult to express??
Posted:
Fri Jan 06, 2012 1:28 pmby vald0drag
where are you tryin to express it bacteria or cells? If it's bacteria, try this product, ir helps:
http://www.sigmaaldrich.com/etc/mediali ... -media.pdfIf it's cells, well you're right, p38 screws up g2 to M transition, and proliferation sucks
Re: p38 difficult to express??
Posted:
Fri Feb 15, 2013 1:04 pmby DNAer