p38 difficult to express??

p38 difficult to express??

Postby meltoumi » Thu Jan 05, 2012 7:36 pm

Anyone else find MAPK14 or p38 alpha difficult to recombinantly express at high titer levels? When p38 is folded and active, it seems to slow cell life cycle and decrease expression. Any thoughts?
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Re: p38 difficult to express??

Postby vald0drag » Fri Jan 06, 2012 1:28 pm

where are you tryin to express it bacteria or cells? If it's bacteria, try this product, ir helps: http://www.sigmaaldrich.com/etc/mediali ... -media.pdf

If it's cells, well you're right, p38 screws up g2 to M transition, and proliferation sucks :(
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Re: p38 difficult to express??

Postby DNAer » Fri Feb 15, 2013 1:04 pm

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